I would check the BIOS to make sure it is set to boot from the correct drive. If it was trying to boot from the litey connected to your add on card, it might tell you to insert a boot disc??
Double check your jumper settings. Some drives have one setting for master with slave, and another for single drive (master with no slave). In other words there is quite often a jumper setting that is used when the drive is by itself on the cable.
"All Bibles are man made" - Thomas Edison